Differential expression profile analysis of DNA damage repair genes in CD133⁺/CD133^- colorectal cancer cells.

ABSTRACT: The present study examined differential expression levels of DNA damage repair genes in COLO 205 colorectal cancer cells, with the aim of identifying novel biomarkers for the molecular diagnosis and treatment of colorectal cancer. COLO 205-derived cell spheres were cultured in serum-free medium supplemented with cell factors, and CD133⁺/CD133^- cells were subsequently sorted using an indirect CD133 microbead kit. In vitro differentiation and tumorigenicity assays in BABA/c nude mice were performed to determine whether the CD133⁺ cells also possessed stem cell characteristics, in addition to the COLO 205 and CD133^- cells. RNA sequencing was employed for the analysis of differential gene expression levels at the mRNA level, which was determin

SUBMITTER: Lu Y 

PROVIDER: S-EPMC5530084 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature