NanoSim: nanopore sequence read simulator based on statistical characterization.

ABSTRACT:

Background

The MinION sequencing instrument from Oxford Nanopore Technologies (ONT) produces long read lengths from single-molecule sequencing - valuable features for detailed genome characterization. To realize the potential of this platform, a number of groups are developing bioinformatics tools tuned for the unique characteristics of its data. We note that these development efforts would benefit from a simulator software, the output of which could be used to benchmark analysis tools.

Results

Here, we introduce NanoSim, a fast and scalable read simulator that captures the technology-specific features of ONT data and allows for adjustments upon improvement of nanopore sequencing technology. The first step of NanoSim is read characterization, which provides a comprehensive a

SUBMITTER: Yang C 

PROVIDER: S-EPMC5530317 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature