Vitamin D receptor expression and associated gene signature in tumour stromal fibroblasts predict clinical outcome in colorectal cancer.

ABSTRACT:

Objective

Colorectal cancer (CRC) is a major health concern. Vitamin D deficiency is associated with high CRC incidence and mortality, suggesting a protective effect of vitamin D against this disease. Given the strong influence of tumour stroma on cancer progression, we investigated the potential effects of the active vitamin D metabolite 1α,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) on CRC stroma.

Design

Expression of vitamin D receptor (VDR) and two 1,25(OH)₂D₃ target genes was analysed in 658 patients with CRC with prolonged clinical follow-up. 1,25(OH)₂D₃ effects on primary cultures of patient-derived colon normal fibroblasts (NFs) and cancer-associated fibroblasts (CAFs) were studied using collagen g

SUBMITTER: Ferrer-Mayorga G 

PROVIDER: S-EPMC5530491 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature