Comparative analysis of targeted long read sequencing approaches for characterization of a plant's immune receptor repertoire.

ABSTRACT:

Background

The Oxford Nanopore Technologies MinION™ sequencer is a small, portable, low cost device that is accessible to labs of all sizes and attractive for in-the-field sequencing experiments. Selective breeding of crops has led to a reduction in genetic diversity, and wild relatives are a key source of new genetic resistance to pathogens, usually via NLR immune receptor-encoding genes. Recent studies have demonstrated how crop NLR repertoires can be targeted for sequencing on Illumina or PacBio (RenSeq) and the specific gene conveying pathogen resistance identified.

Results

Sequence yields per MinION run are lower than Illumina, making targeted resequencing an efficient approach. While MinION generates long reads similar to PacBio it doesn't generate the highly accurate

SUBMITTER: Giolai M 

PROVIDER: S-EPMC5530509 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature