Culturing and transcriptome profiling of progenitor-like colonies derived from adult mouse pancreas.

ABSTRACT:

Background

Transplantation of insulin-producing cells is considered an important diabetes therapy. Many research studies have shown that insulin-producing cells can be derived from the in-vitro cultured pancreatic colonies with self-renewal ability and multilineage potential. Even though these progenitor-like colonies have been prepared from adult pancreas cells, the efficient culture method is hardly established and regulation of the colonies is rarely known. We confirmed previously that single cells acquired from adult mouse pancreas could form cyst-like colonies in a 3D semi-solid system containing Matrigel and methylcellulose. These colonies could be passaged continuously without losing progenitor-like capacity. In the previous culturing system, however, conditioned medium from

SUBMITTER: Ma D 

PROVIDER: S-EPMC5530554 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature