Genome-wide screen for genes involved in eDNA release during biofilm formation by Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is a leading cause of both nosocomial and community-acquired infection. Biofilm formation at the site of infection reduces antimicrobial susceptibility and can lead to chronic infection. During biofilm formation, a subset of cells liberate cytoplasmic proteins and DNA, which are repurposed to form the extracellular matrix that binds the remaining cells together in large clusters. Using a strain that forms robust biofilms in vitro during growth under glucose supplementation, we carried out a genome-wide screen for genes involved in the release of extracellular DNA (eDNA). A high-density transposon insertion library was grown under biofilm-inducing conditions, and the relative frequency of insertions was compared between genomic DNA (gDNA) collected from cells in

SUBMITTER: DeFrancesco AS 

PROVIDER: S-EPMC5530685 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature