Radiative decay engineering 8: Coupled emission microscopy for lens-free high-throughput fluorescence detection.

ABSTRACT: Fluorescence spectroscopy and imaging are now used throughout the biosciences. Fluorescence microscopes, spectrofluorometers, microwell plate readers and microarray imagers all use multiple optical components to collect, redirect and focus the emission onto single point or array imaging detectors. For almost all biological samples, except those with regular nanoscale features, emission occurs in all directions. With the exception of complex microscope objectives with large collection angles (NA ≤ 0.5), all these instruments collect only a small fraction of the total emission. Because of the increasing knowledge base on fluorophores within near-field (<200 nm) distances from plasmonic and photonic structures we can anticipate the development of compact devices in which the sample to be dete

SUBMITTER: Zhu L 

PROVIDER: S-EPMC5530754 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature