Proteomic analysis of human follicular fluid associated with successful in vitro fertilization.

ABSTRACT:

Background

Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome.

Methods

To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF.

Results

A total of 219 unique high-confidence (False Discovery Rate (FDR) < 0.01) HFF proteins were identified by searching the reviewed Swiss-Prot human database (20,183 sequences), and MS data were further verified by wes

SUBMITTER: Shen X 

PROVIDER: S-EPMC5530927 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature