The difference in expression of long noncoding RNAs in rat semen induced by high-fat diet was associated with metabolic pathways.

ABSTRACT:

Background

Obesity, a common metabolic disease, is a known cause of male infertility due to its associated health risk. Long noncoding RNAs (lncRNAs) have also been reported to be associated with male reproductive diseases; however, their role in the association between high-fat diet-induced obesity (DIO) and male reproduction remains unclear.

Methods

We used microarray analysis to compare the expression levels of lncRNAs and mRNAs in the spermatozoa of rats with DIO and normal rats. We selected a few lncRNAs that were obviously up-regulated or down-regulated, and then used RT-PCR to verify the accuracy of their expression. We then performed a functional enrichment analysis of the differentially expressed mRNAs using gene ontology and pathway analysis. Finally, target gene p

SUBMITTER: An T 

PROVIDER: S-EPMC5530988 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature