Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo.

ABSTRACT: Super-resolution light microscopy, correlative light and electron microscopy, and volume electron microscopy are revolutionising the way in which biological samples are examined and understood. Here, we combine these approaches to deliver super-accurate correlation of fluorescent proteins to cellular structures. We show that YFP and GFP have enhanced blinking properties when embedded in acrylic resin and imaged under partial vacuum, enabling in vacuo single molecule localisation microscopy. In conventional section-based correlative microscopy experiments, the specimen must be moved between imaging systems and/or further manipulated for optimal viewing. These steps can introduce undesirable alterations in the specimen, and complicate correlation between imaging modalities. We avoided these

SUBMITTER: Peddie CJ 

PROVIDER: S-EPMC5531056 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature