Multidimensional chemical control of CRISPR-Cas9.

ABSTRACT: Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.

SUBMITTER: Maji B 

PROVIDER: S-EPMC5531067 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature