Proteolytic processing of the L-type Ca ²⁺ channel alpha ₁1.2 subunit in neurons.

ABSTRACT: Background: The L-type Ca2+ channel Cav1.2 is a prominent regulator of neuronal excitability, synaptic plasticity, and gene expression. The central element of Cav1.2 is the pore-forming α ₁1.2 subunit. It exists in two major size forms, whose molecular masses have proven difficult to precisely determine. Recent work suggests that α ₁1.2 is proteolytically cleaved between the second and third of its four pore-forming domains (Michailidis et al,. 2014). Methods: To better determine the apparent molecular masses (M _R)of the α ₁1.2 size forms, extensive systematic immunoblotting of brain tissue as well as full length and C-terminally truncated α ₁1.2 expressed in HEK293 cells was conducted using six different region-sp

SUBMITTER: Buonarati OR 

PROVIDER: S-EPMC5531164 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature