Establishment and genomic characterization of primary salivary duct carcinoma cell line.

ABSTRACT:

Objectives

To develop and characterize in vitro salivary duct carcinoma as a surrogate for functional studies.

Materials and methods

Cells were dispersed from tumor tissue fragments under sterile conditions in RPMI media. Disassociated cells were cultivated, immortalized with hTERT and propagated for more than 100 passages. Morphologic, linage, cytogenetic and genomic analyses were performed on different passages of cell line and primary tumor. Soft agar growth was performed.

Results

Analysis of cytomorphologic features, growth characteristics and lineage specific markers expression confirmed the epithelial derivation and the neoplastic nature of the cell line. DNA STRs analysis showed identical match of both cell line and primary tumor. Cultivated cells expressed And

SUBMITTER: Li J 

PROVIDER: S-EPMC5531198 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature