Circulating microRNAs and association with methacholine PC20 in the Childhood Asthma Management Program (CAMP) cohort.

ABSTRACT:

Introduction

Circulating microRNAs (miRNA) are promising biomarkers for human diseases. Our study hypothesizes that circulating miRNA would reveal candidate biomarkers related to airway hyperresponsiveness (AHR) and provide biologic insights into asthma epigenetic influences.

Methods

Serum samples obtained at randomization for 160 children in the Childhood Asthma Management Program were profiled using a TaqMan miRNA array set. The association of the isolated miRNA with methacholine PC20 was assessed. Network and pathway analyses were performed. Functional validation of two significant miRNAs was performed in human airway smooth muscle cells (HASMs).

Results

Of 155 well-detected circulating miRNAs, eight were significantly associated with PC20 with the strongest associ

SUBMITTER: Davis JS 

PROVIDER: S-EPMC5531511 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature