Monitoring focal adhesion kinase phosphorylation dynamics in live cells.

ABSTRACT: Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

SUBMITTER: Damayanti NP 

PROVIDER: S-EPMC5531600 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature