The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis.

ABSTRACT: MicroRNA cluster mirn23a has previously been shown to promote myeloid development at the expense of lymphoid development in overexpression and knockout mouse models. This polarization is observed early in hematopoietic development, with an increase in common lymphoid progenitors (CLPs) and a decrease in all myeloid progenitor subsets in adult bone marrow. The pool size of multipotential progenitors (MPPs) is unchanged; however, in this report we observe by flow cytometry that polarized subsets of MPPs are changed in the absence of mirn23a. Additionally, in vitro culture of MPPs and sorted MPP transplants showed that these cells have decreased myeloid and increased lymphoid potential in vitro and in vivo. We investigated the mechanism by which mirn23a regulates hematopoietic differentiation

SUBMITTER: Kurkewich JL 

PROVIDER: S-EPMC5531666 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature