Evolution, expression and association of the chemosensory protein genes with the outbreak phase of the two main pest locusts.

ABSTRACT: We analyze the evolutionary relationships and expression patterns of the large set of genes for chemosensory proteins (CSPs) in the two main pest locusts. We used the available transcriptome and genome data to infer the number of genes using BLAST searches and sequence similarity matrices. Maximum likelihood phylogenies revealed the relationships between these CSPs and CSPs from several arthropods. RNAseq and qPCR allowed associating CSPs to locust phases. Crossing the phylogenetic and expression data allowed us to deduce homologies and conservation of the involvement in the phase change. We confirm that Locusta migratoria has at least 58 CSP gene copies, only five of which lack evidence of expression, and we reveal that Schistocerca gregaria has at least 42 expressed CSP genes. Both speci

SUBMITTER: Martin-Blazquez R 

PROVIDER: S-EPMC5532218 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature