Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture.

ABSTRACT: Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells. The device was characterized in terms of cell retention capacity, biocompatibility, scalability, and long-term reliability. This technology was demonstrated using a high concentration (>20 million ce

SUBMITTER: Kwon T 

PROVIDER: S-EPMC5532224 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature