A pilot study demonstrating the altered gut microbiota functionality in stable adults with Cystic Fibrosis.

ABSTRACT: Cystic Fibrosis (CF) and its treatment result in an altered gut microbiota composition compared to non-CF controls. However, the impact of this on gut microbiota functionality has not been extensively characterised. Our aim was to conduct a proof-of-principle study to investigate if measurable changes in gut microbiota functionality occur in adult CF patients compared to controls. Metagenomic DNA was extracted from faecal samples from six CF patients and six non-CF controls and shotgun metagenomic sequencing was performed on the MiSeq platform. Metabolomic analysis using gas chromatography-mass spectrometry was conducted on faecal water. The gut microbiota of the CF group was significantly different compared to the non-CF controls, with significantly increased Firmicutes and decreased Bact

SUBMITTER: Fouhy F 

PROVIDER: S-EPMC5532234 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature