"High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology".

ABSTRACT: The tissue-specific etiology of aging and stress has been elusive due to limitations in data processing of current techniques. Despite that many techniques are high-throughput, they usually use singular features of the data (e.g. whole fluorescence). One technology at the nexus of fluorescence-based screens is large particle flow cytometry ("biosorter"), capable of recording positional fluorescence and object granularity information from many individual live animals. Current processing of biosorter data, however, do not integrate positional information into their analysis and data visualization. Here, we present a bioanalytical platform for the quantification of positional information ("longitudinal profiling") of C. elegans, which we posit embodies the benefits of both high-throughput scr

SUBMITTER: Daniele JR 

PROVIDER: S-EPMC5532364 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature