Reduced Erg Dosage Impairs Survival of Hematopoietic Stem and Progenitor Cells.

ABSTRACT: ERG, an ETS family transcription factor frequently overexpressed in human leukemia, has been implicated as a key regulator of hematopoietic stem cells. However, how ERG controls normal hematopoiesis, particularly at the stem and progenitor cell level, and how it contributes to leukemogenesis remain incompletely understood. Using homologous recombination, we generated an Erg knockdown allele (Erg^kd ) in which Erg expression can be conditionally restored by Cre recombinase. Erg^kd/kd animals die at E10.5-E11.5 due to defects in endothelial and hematopoietic cells, but can be completely rescued by Tie2-Cre-mediated restoration of Erg in these cells. In Erg^kd/+ mice, ∼40% reduction in Erg dosage perturbs both fetal liver and bone marrow hematopoiesis by reducing

SUBMITTER: Xie Y 

PROVIDER: S-EPMC5532742 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature