HLA-restricted epitope identification and detection of functional T cell responses by using MHC-peptide and costimulatory microarrays.

ABSTRACT: Identification of T cell epitopes is a vital but often slow and difficult step in studying the immune response to infectious agents and autoantigens. We report a spatially addressable technique for screening large numbers of T cell epitopes for both specific antigen recognition and functional activity induced. This system uses microarrays of immobilized, recombinant MHC-peptide complexes, costimulatory molecules, and cytokine-capture antibodies. The array elements act as synthetic antigen-presenting cells and specifically elicit T cell responses, including adhesion, secretion of cytokines, and modulation of surface markers. The method allows facile identification of pertinent T cell epitopes in a large number of candidates and simultaneous determination of the functional outcome of the int

SUBMITTER: Stone JD 

PROVIDER: S-EPMC553304 | biostudies-literature | 2005 Mar

REPOSITORIES: biostudies-literature