Tissue-Specific Cell Cycle Indicator Reveals Unexpected Findings for Cardiac Myocyte Proliferation.

ABSTRACT:

Rationale

Discerning cardiac myocyte cell cycle behavior is challenging owing to commingled cell types with higher proliferative activity.

Objective

To investigate cardiac myocyte cell cycle activity in development and the early postnatal period.

Methods and results

To facilitate studies of cell type-specific proliferation, we have generated tissue-specific cell cycle indicator BAC transgenic mouse lines. Experiments using embryonic fibroblasts from CyclinA2-LacZ-floxed-EGFP, or CyclinA2-EGFP mice, demonstrated that CyclinA2-βgal and CyclinA2-EGFP were expressed from mid-G1 to mid-M phase. Using Troponin T-Cre;CyclinA2-LacZ-EGFP mice, we examined cardiac myocyte cell cycle activity during embryogenesis and in the early postnatal period. Our data demonstrated that righ

SUBMITTER: Hirai M 

PROVIDER: S-EPMC5533092 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature