Addressing preferred specimen orientation in single-particle cryo-EM through tilting.

ABSTRACT: We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.

SUBMITTER: Tan YZ 

PROVIDER: S-EPMC5533649 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature