Embryo development after mitochondrial supplementation from induced pluripotent stem cells.

ABSTRACT:

Purpose

The purpose of this study was to evaluate the effects of mitochondrial supplementation (MS) on early embryonic development and to assess the safety of MS treatments using induced pluripotent stem cells (iPSCs) as the mitochondrial donor.

Methods

In this study, we evaluated the effect of MS on early embryonic development using induced pluripotent stem cells (iPSCs) as the donor. Mouse zygotes were injected with either mitochondria from iPSCs or a vehicle solution. Several parameters were evaluated, including the rates of blastocyst formation and implantation, the weight of E13.5 embryos and placentas, the distribution of the donor mitochondrial DNA (mtDNA), and the pattern of methylation in the differentially methylated regions (DMRs) of the H19 and Snrpn genes.

Re

SUBMITTER: Li R 

PROVIDER: S-EPMC5533679 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature