Observed crowding effects on Mycobacterium tuberculosis 2-trans-enoyl-ACP (CoA) reductase enzyme activity are not due to excluded volume only.

ABSTRACT: The cellular milieu is a complex and crowded aqueous solution. Macromolecular crowding effects are commonly studied in vitro using crowding agents. The aim of the present study was to evaluate the effects, if any, of macromolecular synthetic crowding agents on the apparent steady-state kinetic parameters (K _m , k _cat , and k _cat /K _m ) of Mycobacterium tuberculosis 2-trans-enoyl-ACP (CoA) reductase (InhA). Negligible effects on InhA activity were observed for ficoll 70, ficoll 400 and dextran 70. A complex effect was observed for PEG 6000. Glucose and sucrose showed, respectively, no effect on InhA activity and decreased k _cat /K _m for NADH and k _cat for 2-trans-dodecenoyl-CoA. Molecular dynamics results suggest t

SUBMITTER: Rotta M 

PROVIDER: S-EPMC5533716 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature