Mapping the chromatin landscape and Blimp1 transcriptional targets that regulate trophoblast differentiation.

ABSTRACT: Trophoblast stem cells (TSCs) give rise to specialized cell types within the placenta. However, the regulatory mechanisms that guide trophoblast cell fate decisions during placenta development remain ill defined. Here we exploited ATAC-seq and transcriptional profiling strategies to describe dynamic changes in gene expression and chromatin accessibility during TSC differentiation. We detect significantly increased chromatin accessibility at key genes upregulated as TSCs exit from the stem cell state. However, downregulated gene expression is not simply due to the loss of chromatin accessibility in proximal regions. Additionally, transcriptional targets recognized by the zinc finger transcriptional repressor Prdm1/Blimp1, an essential regulator of placenta development, were identified in Ch

SUBMITTER: Nelson AC 

PROVIDER: S-EPMC5533796 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature