Efficiency of ddRAD target enriched sequencing across spiny rock lobster species (Palinuridae: Jasus).

ABSTRACT: Double digest restriction site-associated DNA sequencing (ddRADseq) and target capture sequencing methods are used to explore population and phylogenetic questions in non-model organisms. ddRADseq offers a simple and reliable protocol for population genomic studies, however it can result in a large amount of missing data due to allelic dropout. Target capture sequencing offers an opportunity to increase sequencing coverage with little missing data and consistent orthologous loci across samples, although this approach has generally been applied to conserved markers for deeper evolutionary questions. Here, we combine both methods to generate high quality sequencing data for population genomic studies of all marine lobster species from the genus Jasus. We designed probes based on ddRADseq lib

SUBMITTER: Souza CA 

PROVIDER: S-EPMC5533801 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature