Dynamics of plasma membrane surface related to the release of extracellular vesicles by mesenchymal stem cells in culture.

ABSTRACT: Extracellular vesicles (exosomes and shedding vesicles) released by mesenchymal stem cells (MSCs) are regarded as a storable, cell-free alternative with comparable therapeutic potential to their parent cells. Shedding vesicles originate as bulges on the cell surface but little is known about their turnover or how their formation can be stimulated. We have used atomic force microscopy (AFM) to follow the formation dynamics of bulges in living adipose tissue-derived MSCs. AFM images showed that, in general, MSCs present hundreds of nanosized protrusions on their surface with life spans of 10-20 min. Scanning electron microscopy confirmed those images and showed that bulges are also formed on filamentous processes. Extracellular vesicles deposited on the culture surface have comparable sizes

SUBMITTER: Casado S 

PROVIDER: S-EPMC5533874 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature