Identifying genomic targets for protein over-expression by "omics" analysis of Quiescent Escherichia coli cultures.

ABSTRACT:

Background

A cellular stress response is triggered upon induction of recombinant protein expression which feedback inhibits both growth as well as protein synthesis. In order to separate these two effects, it was decided to study "quiescent cultures" which continue to be metabolically active and express recombinant proteins even after growth cessation. The idea was to identify and up-regulate genes which are responsible for protein synthesis in the absence of growth. This would ensure that, even if growth were adversely affected post induction, there would be no attendant reduction in the protein expression capability of the cells. This strategy allowed us to design host strains, which did not grow better post induction but had significantly higher levels of protein expression.

SUBMITTER: Mahalik S 

PROVIDER: S-EPMC5534100 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature