Identification of Anaplasma ovis appendage-associated protein (AAAP) for development of an indirect ELISA and its application.

ABSTRACT:

Background

Ovine anaplasmosis is a tick-borne disease that is caused by Anaplasma ovis in sheep and goats. The pathogen is widely distributed in tropical and subtropical regions of the world. At present, diagnosis of the disease mainly depends on microscopy or nucleic acid based molecular tests, although a few serological tests have been applied for the detection of A. ovis infection.

Results

Here we describe the identification of an A. ovis protein that is homologous to the A. marginale appendage-associated protein (AAAP). We expressed a recombinant fragment of this protein for the development of an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of A. ovis. Anaplasma ovis-positive serum showed specific reactivity to recombinantly expressed AAAP (rAAAP)

SUBMITTER: Wang Z 

PROVIDER: S-EPMC5534110 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature