Cocoa polyphenols and fiber modify colonic gene expression in rats.

ABSTRACT:

Purpose

Cocoa intake has been associated with health benefits, improving cardiovascular function and metabolism, as well as modulating intestinal immune function. The aim of this study was to take an in-depth look into the mechanisms affected by the cocoa intake by evaluating the colonic gene expression after nutritional intervention, and to ascertain the role of the fiber of cocoa in these effects.

Methods

To achieve this, Wistar rats were fed for 3 weeks with either a reference diet, a diet containing 10 % cocoa (C10), a diet based on cocoa fiber (CF) or a diet containing inulin (I). At the end of the study, colon was excised to obtain the RNA to evaluate the differential gene expression by microarray. Results were validated by RT-PCR.

Results

The C10 group was the

SUBMITTER: Massot-Cladera M 

PROVIDER: S-EPMC5534200 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature