The Fungal Frontier: A Comparative Analysis of Methods Used in the Study of the Human Gut Mycobiome.

ABSTRACT: The human gut is host to a diverse range of fungal species, collectively referred to as the gut "mycobiome". The gut mycobiome is emerging as an area of considerable research interest due to the potential roles of these fungi in human health and disease. However, there is no consensus as to what the best or most suitable methodologies available are with respect to characterizing the human gut mycobiome. The aim of this study is to provide a comparative analysis of several previously published mycobiome-specific culture-dependent and -independent methodologies, including choice of culture media, incubation conditions (aerobic versus anaerobic), DNA extraction method, primer set and freezing of fecal samples to assess their relative merits and suitability for gut mycobiome analysis. There wa

SUBMITTER: Huseyin CE 

PROVIDER: S-EPMC5534473 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature