Coupled feedback loops control the stimulus-dependent dynamics of the yeast transcription factor Msn2.

ABSTRACT: Information about environmental stimuli often can be encoded by the dynamics of signaling molecules or transcription factors. In the yeast Saccharomyces cerevisiae, different types of stresses induce distinct nuclear translocation dynamics of the general stress-responsive transcription factor Msn2, but the underlying mechanisms remain unclear. Using deterministic and stochastic modeling, we reproduced in silico the different dynamic responses of Msn2 to glucose limitation and osmotic stress observed in vivo and found that a positive feedback loop on protein kinase A mediated by the AMP-activated protein kinase Snf1 is coupled with a negative feedback loop to generate the characteristic pulsatile dynamics of Msn2. The model predicted that the stimulus-specific positive

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC5535011 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature