The transcriptional repressor GATAD2B mediates progesterone receptor suppression of myometrial contractile gene expression.

ABSTRACT: The mechanisms whereby progesterone (P₄), acting via the progesterone receptor (PR), inhibits proinflammatory/contractile gene expression during pregnancy are incompletely defined. Using immortalized human myometrial (hTERT-HM) cells stably expressing wild-type PR-A or PR-B (PR_WT), we found that P₄ significantly inhibited IL-1β induction of the NF-κB target genes, COX-2 and IL-8 P₄-PR_WT transrepression occurred at the level of transcription initiation and was mediated by decreased recruitment of NF-κB p65 and RNA polymerase II to COX-2 and IL-8 promoters. However, in cells stably expressing a PR-A or PR-B DNA-binding domain mutant (PR_mDBD), P₄-mediated transrepression was significantly

SUBMITTER: Chen CC 

PROVIDER: S-EPMC5535031 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature