Estrogen receptor α activation enhances its cell surface localization and improves myocardial redox status in ovariectomized rats.

ABSTRACT:

Aims

Little is known about the role of subcellular trafficking of estrogen receptor (ER) subtypes in the acute estrogen (E₂)-mediated alleviation of oxidative stress. We tested the hypothesis that ERα migration to the cardiac myocyte membrane mediates the acute E₂-dependent improvement of cellular redox status.

Main methods

Myocardial distribution of subcellular ERα, ERβ and G-protein coupled estrogen receptor (GPER) was determined in proestrus sham-operated (SO) and in ovariectomized (OVX) rats, acutely treated with E₂ (1μg/kg) or a selective ERα (PPT), ERβ (DPN) or GPER (G1) agonist (10μg/kg), by immunofluorescence and Western blot. We measured ROS and malondialdehyde (MDA) levels, and catalase and superoxide dismutase (SOD) activities to e

SUBMITTER: Steagall RJ 

PROVIDER: S-EPMC5535783 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature