Clonal fate mapping quantifies the number of haematopoietic stem cells that arise during development.

ABSTRACT: Haematopoietic stem cells (HSCs) arise in the developing aorta during embryogenesis. The number of HSC clones born has been estimated through transplantation, but experimental approaches to assess the absolute number of forming HSCs in a native setting have remained challenging. Here, we applied single-cell and clonal analysis of HSCs in zebrafish to quantify developing HSCs. Targeting creER^T2 in developing cd41:eGFP⁺ HSCs enabled long-term assessment of their blood contribution. We also applied the Brainbow-based multicolour Zebrabow system with drl:creER^T2 that is active in early haematopoiesis to induce heritable colour barcoding unique to each HSC and its progeny. Our findings reveal that approximately 21 HSC clones exist prior to HSC emergence and 30 c

SUBMITTER: Henninger J 

PROVIDER: S-EPMC5535785 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature