CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.

ABSTRACT: Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray crystallography as a pipeline for obtaining the required structures. We have examined the potential of single-particle cryoEM for determining the structure of influenza-virus hemagglutinin (HA):single-chain variable-domain fragment complexes, by studying a complex we failed to crystallize in pursuing an extended project on the human immune response to influenza vaccines.The result shows that a combination of cryoEM and molecular modeling can yield details of the antigen-antibody interface, although small variation in the twist of the rod-likeHA trimer limited the overall resolution to about 4.5Å.C

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5535819 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature