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Reply to the Letter to the Editor by A. Schwelm and S. Neuhauser: "Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates".

ABSTRACT: The authors of Laila et al. [1] would like thank to the readers (A. Schwelm and S. Neuhauser) for submitting a letter requesting the authors to correct ribosomal DNA (rDNA) sequences of 11 Korean Plasmodiophora bassicae isolates at the 3'-end.[...].

SUBMITTER: Laila R

PROVIDER: S-EPMC5535946 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Reply to the Letter to the Editor by A. Schwelm and S. Neuhauser: "Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates".

Laila Rawnak R Park Jong-In JI Robin Arif Hasan Khan AHK Yang Kiwoung K Choi Gyung Ja GJ Nou Ill-Sup IS

International journal of molecular sciences 20170706 7

The authors of Laila et al. [1] would like thank to the readers (A. Schwelm and S. Neuhauser) for submitting a letter requesting the authors to correct ribosomal DNA (rDNA) sequences of 11 Korean Plasmodiophora bassicae isolates at the 3'-end.[...]. ...[more]

PMID: 28684669

Similar Datasets

Letter to the Editor: "Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates".

Project description:In the publication "Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates", Laila et al.[...].

| S-EPMC5535945 | biostudies-literature

Detection of Ribosomal DNA Sequence Polymorphisms in the Protist Plasmodiophora brassicae for the Identification of Geographical Isolates.

Project description:Clubroot is a soil-borne disease caused by the protist Plasmodiophora brassicae (P. brassicae). It is one of the most economically important diseases of Brassica rapa and other cruciferous crops as it can cause remarkable yield reductions. Understanding P. brassicae genetics, and developing efficient molecular markers, is essential for effective detection of harmful races of this pathogen. Samples from 11 Korean field populations of P. brassicae (geographic isolates), collected from nine different locations in South Korea, were used in this study. Genomic DNA was extracted from the clubroot-infected samples to sequence the ribosomal DNA. Primers and probes for P. brassicae were designed using a ribosomal DNA gene sequence from a Japanese strain available in GenBank (accession number AB526843; isolate NGY). The nuclear ribosomal DNA (rDNA) sequence of P. brassicae, comprising 6932 base pairs (bp), was cloned and sequenced and found to include the small subunits (SSUs) and a large subunit (LSU), internal transcribed spacers (ITS1 and ITS2), and a 5.8s. Sequence variation was observed in both the SSU and LSU. Four markers showed useful differences in high-resolution melting analysis to identify nucleotide polymorphisms including single- nucleotide polymorphisms (SNPs), oligonucleotide polymorphisms, and insertions/deletions (InDels). A combination of three markers was able to distinguish the geographical isolates into two groups.

| S-EPMC5297718 | biostudies-literature

Reply to Letter to the Editor.