Rapid and Direct VHH and Target Identification by Staphylococcal Surface Display Libraries.

ABSTRACT: Unbiased and simultaneous identification of a specific antibody and its target antigen has been difficult without prior knowledge of at least one interaction partner. Immunization with complex mixtures of antigens such as whole organisms and tissue extracts including tumoral ones evokes a highly diverse immune response. During such a response, antibodies are generated against a variety of epitopes in the mixture. Here, we propose a surface display design that is suited to simultaneously identify camelid single domain antibodies and their targets. Immune libraries of single-domain antigen recognition fragments from camelid heavy chain-only antibodies (VHH) were attached to the peptidoglycan of Gram-positive Staphylococcus aureus employing its endogenous housekeeping sortase enzyme. The sort

SUBMITTER: Cavallari M 

PROVIDER: S-EPMC5535997 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature