Prediction of Protein Hotspots from Whole Protein Sequences by a Random Projection Ensemble System.

ABSTRACT: Hotspot residues are important in the determination of protein-protein interactions, and they always perform specific functions in biological processes. The determination of hotspot residues is by the commonly-used method of alanine scanning mutagenesis experiments, which is always costly and time consuming. To address this issue, computational methods have been developed. Most of them are structure based, i.e., using the information of solved protein structures. However, the number of solved protein structures is extremely less than that of sequences. Moreover, almost all of the predictors identified hotspots from the interfaces of protein complexes, seldom from the whole protein sequences. Therefore, determining hotspots from whole protein sequences by sequence information alone is urgen

SUBMITTER: Jiang J 

PROVIDER: S-EPMC5536031 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature