Colorful Packages: Encapsulation of Fluorescent Proteins in Complex Coacervate Core Micelles.

ABSTRACT: Encapsulation of proteins can be beneficial for food and biomedical applications. To study their biophysical properties in complex coacervate core micelles (C3Ms), we previously encapsulated enhanced green fluorescent protein (EGFP) and its monomeric variant, mEGFP, with the cationic-neutral diblock copolymer poly(2-methyl-vinyl-pyridinium)_n-b-poly(ethylene-oxide)_m (P2MVP_n-b-PEO_m) as enveloping material. C3Ms with high packaging densities of fluorescent proteins (FPs) were obtained, resulting in a restricted orientational freedom of the protein molecules, influencing their structural and spectral properties. To address the generality of this behavior, we encapsulated seven FPs with P2MVP₄₁-b-PEO₂₀₅ and P

SUBMITTER: Nolles A 

PROVIDER: S-EPMC5536045 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature