Flux balance analysis predicts Warburg-like effects of mouse hepatocyte deficient in miR-122a.

ABSTRACT: The liver is a vital organ involving in various major metabolic functions in human body. MicroRNA-122 (miR-122) plays an important role in the regulation of liver metabolism, but its intrinsic physiological functions require further clarification. This study integrated the genome-scale metabolic model of hepatocytes and mouse experimental data with germline deletion of Mir122a (Mir122a-/-) to infer Warburg-like effects. Elevated expression of MiR-122a target genes in Mir122a-/-mice, especially those encoding for metabolic enzymes, was applied to analyze the flux distributions of the genome-scale metabolic model in normal and deficient states. By definition of the similarity ratio, we compared the flux fold change of the genome-scale metabolic model computational results and metabolomic pro

SUBMITTER: Wu HQ 

PROVIDER: S-EPMC5536358 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature