An optical labeling-based proliferation assay system reveals the paracrine effect of interleukin-6 in breast cancer.

ABSTRACT: Proliferation analysis is one of the basic approaches to characterize various cell types. In conventional cell proliferation assays, the same sample cannot be observed over time, nor can a specific group within a heterogeneous population of cells, for example, cancerous cells, be analyzed separately. To overcome these limitations, we established an optical labeling-based proliferation assay system with the Kaede protein, whose fluorescence can be irreversibly photo converted from green to red by irradiation. After a single non-toxic photoconversion event, the intensity of red fluorescence in each cell is reduced by cell division. From this, we developed a simple method to quantify cell proliferation by monitoring reduction of red fluorescence over time. This study shows that the optical la

SUBMITTER: Itou J 

PROVIDER: S-EPMC5536966 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature