Applying systems-level spectral imaging and analysis to reveal the organelle interactome.

ABSTRACT: The organization of the eukaryotic cell into discrete membrane-bound organelles allows for the separation of incompatible biochemical processes, but the activities of these organelles must be coordinated. For example, lipid metabolism is distributed between the endoplasmic reticulum for lipid synthesis, lipid droplets for storage and transport, mitochondria and peroxisomes for β-oxidation, and lysosomes for lipid hydrolysis and recycling. It is increasingly recognized that organelle contacts have a vital role in diverse cellular functions. However, the spatial and temporal organization of organelles within the cell remains poorly characterized, as fluorescence imaging approaches are limited in the number of different labels that can be distinguished in a single image. Here we present a sys

SUBMITTER: Valm AM 

PROVIDER: S-EPMC5536967 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature