Cell-free DNA copy number variations in plasma from colorectal cancer patients.

ABSTRACT: To evaluate the clinical utility of cell-free DNA (cfDNA), we performed whole-genome sequencing to systematically examine plasma cfDNA copy number variations (CNVs) in a cohort of patients with colorectal cancer (CRC, n = 80), polyps (n = 20), and healthy controls (n = 35). We initially compared cfDNA yield in 20 paired serum-plasma samples and observed significantly higher cfDNA concentration in serum (median = 81.20 ng, range 7.18-500 ng·mL^-1 ) than in plasma (median = 5.09 ng, range 3.76-62.8 ng·mL^-1 ) (P < 0.0001). However, tumor-derived cfDNA content was significantly lower in serum than in matched plasma samples tested. With ~10 million reads per sample, the sequencing-based copy number analysis showed common CNVs in multiple chromosomal regions, including ampli

SUBMITTER: Li J 

PROVIDER: S-EPMC5537711 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature