Precast Gelatin-Based Molds for Tissue Embedding Compatible with Mass Spectrometry Imaging.

ABSTRACT: Preparation of tissue for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) generally involves embedding the tissue followed by freezing and cryosectioning, usually between 5 and 25 μm thick, depending on the tissue type and the analyte(s) of interest. The brain is approximately 60% fat; it therefore lacks rigidity and poses structural preservation challenges during sample preparation. Histological sample preparation procedures are generally transferable to MALDI-MSI; however, there are various limitations. Optimal cutting temperature compound (OCT) is commonly used to embed and mount fixed tissue onto the chuck inside the cryostat during cryosectioning. However, OCT contains potential interferences that are detrimental to MALDI-MSI, while fixation is undesi

SUBMITTER: Gill EL 

PROVIDER: S-EPMC5537722 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature