Development of a high-density linkage map and mapping of the three-pistil gene (Pis1) in wheat using GBS markers.

ABSTRACT:

Background

The wheat mutant line three-pistil (TP) exhibits three pistils per floret. As TP normally has two or three seeds in each of the florets on the same spike, there is the possibility of increasing the number of grains per spike. Therefore, TP is a highly valuable mutant for breeding and for the study of floral development in wheat. To map the three-pistil gene (Pis1), genotyping-by-sequencing single-nucleotide polymorphism (GBS-SNP) data from an F₂ mapping population (CM28 × CM28TP) was used to construct a genetic map that is of significant value.

Results

In the present study, a high-density genetic map of wheat containing 2917 GBS-SNP markers was constructed. Twenty-one linkage groups were resolved, with a total length of 2371.40 cM. The individual chromo

SUBMITTER: Yang Z 

PROVIDER: S-EPMC5537994 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature