Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer.

ABSTRACT: Hybridoma screening is a critical step for antibody discovery, which necessitates prompt identification of potential clones from hundreds to thousands of hybridoma cultures against the desired immunogen. Technical issues associated with ELISA- and flow cytometry-based screening limit accuracy and diminish high-throughput capability, increasing time and cost. Conventional ELISA screening with coated antigen is also impractical for difficult-to-express hydrophobic membrane antigens or multi-chain protein complexes. Here, we demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression. The image cytometry-based high

SUBMITTER: Zhang H 

PROVIDER: S-EPMC5538021 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature