MinION Analysis and Reference Consortium: Phase 2 data release and analysis of R9.0 chemistry.

ABSTRACT:

Background

Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Escherichiacoli K-12 using the R9.0 chemistry, comparing the results with the older R7.3 chemistry.

Methods

We computed the error-rate estimates for insertions, deletions, and mismatches in MinION reads.

Results

Run-time characteristics of the flow cell and run scripts for R9.0 were similar to those observed for R7.3 chemistry, but with an 8-fold increase in bases pe

SUBMITTER: Jain M 

PROVIDER: S-EPMC5538040 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature